Restriction enzymes are used to generate DNA fragments with ends that can be joined to create recombinant DNA; which statement describes this outcome?

• A. They create identical copies of the entire genome.
• B. They join fragments end-to-end automatically.
• C. They cut DNA to create fragments with specific ends suitable for ligation.
• D. They convert DNA into RNA.

Answer: (C)

Restriction enzymes cut DNA at specific sequences, producing fragments with ends that can be joined to other DNA pieces by DNA ligase to form recombinant DNA. This describes creating fragments with compatible ends that can be ligated together to assemble new genetic combinations. They don’t copy the entire genome, they don’t automatically join fragments on their own (ligation is needed), and they don’t convert DNA into RNA.